Polymer paper to detect heart disease
doi:10.1038/nindia.2012.90 Published online 22 June 2012
Researchers have designed a new type of biosensor that can detect minute traces of troponin, a muscle protein linked to heart disease. The researchers made the sensor by coating filter papers with polyaniline, a conducting polymer. Such sensors will be very useful for detecting troponin, a diagnostic marker for heart disease.
Troponin is a complex of three regulatory proteins — troponin C, troponin I and troponin T — that is integral to contraction of skeletal and cardiac muscle. Although its usefulness as a diagnostic marker for various heart disorders is well known, there is a lack of reliable test for measuring troponin levels in the blood.
To devise an easy and cost-effective technique, the researchers coated filter papers with the conducting polymer polyaniline using screen printing and electrochemical deposition. They smeared anti-cardiac troponin I antibodies on the polymer-coated filter papers, which bound to the papers through covalent bonds, and carried out electrochemical studies to measure the efficacy of the resulting biosensor.
The study revealed selective detection of the cardiovascular biomarker troponin I through electrochemical transduction. The researchers utilized these strips of polymer-coated paper for detecting troponin across a wide physiological range (1–100 ng mL–1) with great sensitivity.
The researchers say that the electrochemically coated conducting paper based biosensor could form the basis for a point-of-care diagnostic kit for heart disorders.
- Jagadeesan, K. K. et al. Application of conducting paper for selective detection of troponin. Electrochem. Commun. 20, 71-74 (2012) | Article |